Biomarkers association in airway inflammation in the severity of COPD patients in North Indian population

Authors

  • Seema Singh, Sunita Singh, Supriya Pathak, Sandeep Kumar Singh, Santosh Kumar, Prabhudutta Panda, S. K. Verma, Rishikesh Gupta

Abstract

The role of various biomarkers and its correlation with the severity of disease in Chronic Obstructive Pulmonary Disease (COPD) patients were performed in the current study.

Aims: To assess the level of serum cytokines in the patients of COPD in present study of interleukin-4 (IL-4), interleukin-8 (IL-8) and nitric oxide (NO+), protease neutrophil elastase  (NE) and Superoxide dismutase (SOD)  levels

Settings and Design: In this study, a total of 250 COPD patients and 80 healthy controls were enrolled.

Methods and Material: 5 ml of venous blood samples were taken from all the participants and it was collected in a plain vial and then centrifuged at 3000 rpm for 10 minutes. Serum was isolated for determining the role of IL-8, IL-4, NO+, NE and SOD levels in the COPD patients by appropriate test recommended.

Statistical analysis:Analytical analysis was done by SPSS  software 20.0 version (Chicago, Inc., USA).The p-value<0.05 was considered significant.

Results: Compared to healthy control subjects, the serum levels of IL-8, IL-4, NO+, NE and SOD were significantly higher with p–value <0.001 in COPD patients. Between the severity of the COPD stages,  there was a significant difference in the serum level of IL-8, IL-4, NO+, NE and SOD with a (p-value<0.001) in mild, moderate, severe and very severe COPD stages.

Conclusions:Smoking causes inflammation of lung tissue and also responsible for producing oxidative stress which results in imbalance between oxidant and antioxidants present in lung tissue. Decline in lung function (FEV1% predicted) is a result of this imbalance and inflammation. Hence it can be concluded that measurement of these (IL-4, IL-8, NE, SOD and NO+) biomarkers in serum may impart a adequate point of view to evaluate severity of the disease in COPD patients.

Published

2020-11-01

Issue

Section

Articles