Effect Of Ethanol Extract Of Capsicum sp. On The Protoscolices Of Sheep Hydatid Cysts And Pathogenic Bacteria

Authors

  • Areej Hasan S. Aldhaher*, Shaimaa A. Alsamir, Ghosoon F. Al-Kanaany

Abstract

Background: Hydatidosis is a zoonotic disease that requires surgery as one of the best
methods for treatment, in conjunction with scolicidal agents to prevent the formation of new cystic
echinococcosis. Bacterial infection is another risk in hydatidosis surgery. Due to the side effects of chemical
scolicidal agents, this study aimed to evaluate the effect of ethanol extract of Capsicum sp. on the
protoscolices of hydatid cysts and some pathogenic bacteria species in vitro.
Methods: The hydatid cysts of Echinococcosis sp. were collected from the livers of infected sheep. The
scolicidal effect was determined at different exposure times (5, 10, 15 mins.) and different concentrations (50,
100, 500 mg/ml), and the viability of the protoscolices was detected by 0.1 % eosin staining. The antibacterial
activity of the Capsicum ethanol extract was estimated using the agar well diffusion method against
Staphylococcus aureus and Pseudomonas aeruginosa. The secondary metabolites of ethanol extract were
assessed using several qualitative tests.
Results: The results showed the highest scolicidal efficacy after 15 mins of exposure at all used concentrations
with a 22.4%, 19.8% and 0% survival rate respectively. The highest antibacterial activity was recorded against
Staphylococcus aureus with MIC 125 mg/ml.
Conclusion: Capsicum ethanol extract has a good protoscolicidal effect in a short exposure time and could be
used as a scolicidal agent. However, in vivo studies are necessary to confirm the effect.

Published

2020-03-25

Issue

Section

Articles