Determination of the T-2 Mycotoxin in the Republic of Tatarstan by Enzyme-linked Immunosorbent Assay

Authors

  • I.N. Shtyrov*, A.F. Khasiyatullin, D.R. Sagdeev, A.R. Valiev, O.K. Ermolaeva, S.A. Tanaseva , N.N. Mishina

Abstract

This study provides a picture of the spread of T-2 toxin in the Republic of Tatarstan.
Fungi producing T-2 toxin are widespread in the Republic of Tatarstan due to favorable
climatic conditions for their spread and growth. The high toxicity of the T-2 toxin and its
producers determines the need for its detection in feed and food products. There are a number
of methods for indicating T-2 toxin in feed based on chromatographic analysis, but they are
very complex. Enzyme-linked immunosorbent assay does not require expensive equipment,
special training for employees performing the analysis and long sample preparation, which
meets modern realities when there is a need to work with several dozen types of samples at
once. 1191 samples were examined (maize, wheat, barley, oat and compound feed) from 10
regions of the Republic of Tatarstan for being infected with T-2 toxin. The highest percentage
of T-2 toxin detection was in maize samples. Also, the maximum concentration of T-2 toxin
per sample was detected (298.9 ?g/kg) in maize. The highest concentrations of the T-2 toxin
were detected in samples of maize, wheat and oat from the southern part of the Republic of
Tatarstan. While samples from the northern part of the Republic of Tatarstan have the highest
percentage of T-2 infection with toxin. The minimum detected concentration of T-2 toxin
when using these components was 25.1 ?g/kg.

Published

2020-10-23

Issue

Section

Articles