Aspects of model plant purple false brome (Brachypodium distachyon) propagation in vitro

Authors

  • Nargul Omirbekova, Aizhan Zhussupova*, Tlek Mukhanov, Zhazira Zhunusbaeva, Bibinur Yertaeva, Saule Kenzhebaeva

Abstract

Brachypodium distachyon is a model object phylogenetically closest to commercially valuable cereals: wheat, barley, rice and other, and might possibly be useful in their genetic transformation.  The aim of this work was to increase the yield of morphogenetically stable regenerants from a single Brachypodium distachyon explant in vitro, and to optimize the mode of adaptation for test plants in ex situ conditions. Selection of the Brachypodium distachyon explant and optimization of the composition of the nutrient medium for in vitro cultivation were performed. The callus-forming ability and regenerative potential of explants from generative and vegetative organs of Brachypodium distachyon were evaluated. The results obtained on the selection of explants indicate that the nodal segments of vegetative shoots are optimal for microclonal propagation of Brachypodium distachyon. The shoot-forming ability of the primary explants was about 59%, the average reproduction rate for two passages was 8.7.  As a result of our experiments, we obtained an increased number of regenerated plants from one explant with a high percentage – 49.3-80% of the yield of morphogenetically stable regenerants.

Published

2020-11-01

Issue

Section

Articles