STUDY ON ANTIMICROBIAL ACTIVITIES AND PHYTOCHEMICAL ANALYSIS OF SCURRULA FERRUGINEA LEAVES EXTRACTION ON PATHOGENIC BACTERIA AND FUNGI

Authors

  • A. Azral-Ismawy, P.M. Ridzuan, Shariff Halim, C.N. Abdul Rassip , Salwani Ismail , Nor Iza A. Rahman

Abstract

— Scurrula ferrugineus is a hemi-parasitic plant which can be found in Asia and widely used to
treat several problems like hypertension, snake bite, gastrointestinal problem and Alzheimer’s disease. This
research aim to explore the potential of S. ferrugineus as a broad antimicrobial agent on different pathogenic
bacteria and fungi. Method: The plant sample was soaked into ethanol and extracted by using a rotary
evaporator. Drug sensitivity test and synergistic effect were performed by using discs diffusion to identify
zone of inhibition. One-way ANOVA was carried out by using SPSS to identify significant level of the
result. Gas Chromatography-Mass Spectrometry (GC-MS) was done to evaluate the phytochemical
compounds of this extract. Result: Ethanol extract showed significant antimicrobial activities (p<0.05)
against most of the pathogenic bacteria except S. pyogenes and Candida species. However, S. pyogenes
showed potentiation and significant effect on synergistic effect when combined with standard antibiotic.
Other Gram-positive and negative bacteria showed significant synergism effect (p<0.05) except for S.
aureus, S. epidermidis, S. typhimurium and Candida species. GC-MS result showed 35 phytochemical
compounds were identified in this ethanol extract.Conclusion: The results showed the S. ferrugineus as an
effective antimicrobial agent against most of Gram-positive (S. aureus, S. epidermidis, S. saprophyticus, M.
smegmatis, B.subtilis) and Gram-negative (E. coli, P. mirabilis, K. pneumoniae, E. clocae and S.
typhimurium) except for S. pyogenes which showed no sensitivity towards crude but potentiating effect on
synergistic test. Candida species showed no sensitivity towards both tests

Published

2020-02-28

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Section

Articles