Use of grass bio-waste as a substrate for the cultivation of the edible mushroom Pleurotus akin ostreatus

Abstract

The present research has as objective the use of the biowaste lawn coming from the gardens as a
substrate, which is not properly used, , adapting it according to the balance of the C/N ratio, for the
cultivation of Pleurotus akin ostreatus; For this purpose, it was proceeded to the proliferation of the fungus,
elaboration of the seed in barley grains, inoculum at 5% over 1 kg of four different substrates in
polypropylene bag with five replicas of each one, measurement of the production and nutritional analysis of
the fruitful bodies coming from the substrate of higher productivity. As a result of the production; with the
substrate of C/N equal to 20.37 there was a biological efficiency (EB) of 32.832%, with the substrate of C/N
equal to 30 an EB of 43.441%, with the substrate of C/N equal to 40.13 an EB of 37.441% and, with the
substrate of C/N equal to 94.12 an EB of 8.315%. In conclusion; with 95% confidence, the substrate
composed of 61% grass mixed with 39% C/N sawdust equal to 30, the best EB of 43.441% is obtained and,
with a protein content of 32.940% in the fruiting bodies of the fungus.