In Vitro Effect of Pineaple (Ananas comosus L.Mer) Core extract on Growth of Candida albicans

Abstract

-One of the diseases caused by the fungus Candida albicans is a skin disease where the red or white
area of the skin is accompanied by itching, burning and inflammation. As for anti-fungi that are generally
used like Nystatin, but if consumed in excess can cause side effects such as nausea, vomiting, and diarrhea.
one of pineapple waste in the form of core that has not been used optimally is even more often wasted as
waste, whereas the pineapple core contains several active components, one of which is the enzyme bromelin
which bromelin enzyme can be used as an anti-fungal anti-bacterial and anti-inflammatory. This study aims
to determine the active compound extract of pineapple cores and determine the diameter of the antifungal
inhibition against Candida albicans at concentrations of 80%, 60%, 40% and 20%. Nystatin as a positive
control and DMSO as a negative control. The research method was carried out in vitro by carrying out a
qualitative phytochemical test and inhibitory test using the disc diffusion method. Based on the results of the
phytochemical tests, the active compounds obtained were flavonoids, saponins and tannins. The inhibitory
test results of Candida albicans with extract concentrations of 80%, 60%, 40%, 25%, 20%, 15% and 10%
obtained inhibitory zone diameters respectively 12.3 mm, 10.7 mm, 8.6 mm, 12.3 mm, 8.6 mm and positive
control (nystatin) 24.7 mm. it can be concluded that the ethanol extract of pineapple core has the ability as
an antifungal Candida albicans. Based on the results of the phytochemical tests, the active compounds
obtained were flavonoids, saponins and tannins. The inhibitory test results of Candida albicans with extract
concentrations of 80%, 60%, 40%, 25%, 20%, 15% and 10% obtained inhibitory zone diameters
respectively 12.3 mm, 10.7 mm, 8.6 mm, 12.3 mm, 8.6 mm and positive control (nystatin) 24.7 mm. it can
be concluded that the ethanol extract of pineapple core has the ability as an antifungal Candida albicans.
Based on the results of the phytochemical tests, the active compounds obtained were flavonoids, saponins
and tannins. The inhibitory test results of Candida albicans with extract concentrations of 80%, 60%, 40%,
25%, 20%, 15% and 10% obtained inhibitory zone diameters respectively 12.3 mm, 10.7 mm, 8.6 mm, 12.3
mm, 8.6 mm and positive control (nystatin) 24.7 mm. it can be concluded that the ethanol extract of
pineapple core has the ability as an antifungal Candida albicans.