TEMPERATURE CONTROL OF THERMOCYCLER USING PLC AND HMI

Authors

  • Selvaraj S P, Logasundari V, Kumaresan K

Abstract

Deoxyribonucleic Acid (DNA) amplification is one of the important factors to identify human disease. Amplification can be done by rapidly changing the temperature of the sample with primer in the sequences. The first stage of sequence is denaturation with the temperature range between normal to 95 . Extension of the DNA process from 95  to 56  will be the second stage of the sequence and the third stage of sequence is annealing range between 56  and 76 . In this project, the rapid change in temperature is developed with the Peltier element and Programmable Logic Controller (PLC) based controller. The rapid change in temperature and temperature reversal is required for the thermocycler. So it proposed to implement a Bismuth Telluride based Peltier element. It will absorb heat on one side and expel the other side when electrical supply is passed across the junction of the Peltier element. A load or heat sink is essential for both sides of the element to avoid damage to the Peltier element. A Direct Current (DC) fan is used to cool the process, by absorbing the heat from the heat sink. The temperature control of the Polymerase Chain Reaction (PCR) System is accomplished by PLC programming and Human Machine Interface (HMI).

Published

2020-10-16

Issue

Section

Articles