Antimicrobial, Antioxidant and Cytotoxic Activity of methanolic leaf extract of Putranjiva roxburghii :An in-vitro study
AbstractPutranjiva roxburghii also called as Child’s Amulet tree has been used extensively in traditional medicinal practices. In this study we explore the bioactive abilities of P. roxburghii obtained from Sagar taluk, Shivamogga district, Karnataka. P. roxburghii leaves were subjected to sequential soxhlet extraction, of which the methanolic extract showed highest antimicrobial activity against Bacillus subtilis (ATCC 6633), Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 9027), Escherichia coli (ATCC 8739) and Candida albicans (ATCC 10231). The results indicate that the P. roxburghii leaf methanolic extract has a broad spectrum antimicrobial activity with high susceptibility seen towards gram negative organisms. The MIC value against P. aeruginosa and E. coli was found to be 39.60µg/ml and 19.53µg/ml respectively. The extract was evaluated for its antioxidant activity and detection of potential phytocompounds by GC MS analysis. The methanolic extract showed lower antioxidant activity and GC-MS results revealed presence of certain bioactive compounds like Pentatriacontane, Benzenedicarboxylic acid, 2,4-Ditert-Butylphenol and Neophytadine. In-vitro cytotoxicity of the methanolic extract against MCF-7(Human Breast Carcinoma) showed a toxicity of 39.11±1.97 % at 1000µg/ml.